齐发体育shengwuwulisuowangyanlizuhegongweiminzuhezuojieshi cas12i2 shuangmeiliziyilaide dna qiegejizhi
齐发体育 10yue16ri，wangyanliketizuhezhongguokexuejishudaxuegongweiminketizuhezuodelunwen"structural basis for two metal-ion catalysis of dna cleavage by cas12i2"zai《nature communications》zazhizaixianfabiao。gaigongzuobaodaolecas12i2-crrnahecas12i2-crrna-dnafuhewudejingtijiegou，jieshilecas12i2duidnadeshibieheqiegejizhi，bingshouciguanchadaocrispr-casxitongxiaoyingdanbaideruvccuihuajiegouyuhuoxingzhuangtaidejiegou。gaiyanjiujieguobujinjieshilecas12i2jiangjiednadefenzijili，gengzhongyaodeshiyouzhuyulijiehanyouruvcjiegouyudecasdanbaidecuihuajizhi。
crispr-casxitongshixijunhegujunzhongdehuodexingmianyixitong，qizhong，cas9hecas12ashimuqianzuichangyongdejiyinbianjigongju，ruvcjiegouyushicas9yucas12qiegednadezhongyaocuihuabuwei。yinci，huoderuvcjiegouyutongshijiehediwudnahejinshulizidejiegou，duiyulejieruvc jiegouyudecuihuajizhiyouzhongyaoyiyi。
2018nian12yue，scottketizufaxianv-iyaxingxiaoyingdanbaicas12i，erqiecas12ibimuqianyongyujiyinbianjigongjudecas12ahecas9fenzilianggengxiao，yinciyouwangchengweixindejiyinbianjigongju，juci，yanjiucas12idejiegouhegongnengyouzhongyaoyiyi。
benyanjiubaodaolecas12i2decrrnajiehezhuangtai、zhongziquyupeiduizhuangtai、cuihuazhuangtaisanzhongbutongzhuangtaidegaofenbianlvjiegou。yanjiufaxian，zhiyoudangcrrna:dnapeiduizhangdudayu13 ntshi，cas12i2dehelical-iijiegouyucaihuifashenggouxianggaibian，daozhidiwujiehetongdaodakai，bingjihuoruvcjiegouyu。zhongyaodeshi，zaigaofenbianlvcas12i2-crrna-dnasanyuanfuhewujingtijiegouzhong，ruvcjiegouyutongshijieheleyiduandiwussdnahe2gemeilizi，jieshileruvcjiegouyushuangmeiliziyilaidednaqiegejizhi，zheiyeshishouciguanchadaoruvcjiegouyutongshijiehediwudnahejinshulizi，zhanshileqihuoxingzhuangtaidejiegou。
齐发体育 ciwai，gaigongzuohaixitongdiyanjiulecas12i2deshenghuaxingzhi，jieshilecas12i2qianticrrnajiagongjizhi，bingfaxianqianticrrnadejiagongyingxiangshuangliandnaqiegehuoxing。yanjiuhaifaxian，cas12i2haijuyoupamfeiyilaixingdefeiteyixingdanliandnadeqiegehuoxing；youqudeshi，zhongziquyudewanmeipeiduiduiyushuangliandnadeqiegezhiguanzhongyao，quebushidanliandnaqiegedebiyaotiaojian。gaiyanjiuduiyujiyinbianjihehesuanjiancegongjudekaifayouzhongyaoyiyi。
qifatiyushengwuwulisuowangyanliyanjiuyuan、shengganggaojigongchengshihezhongguokexuejishudaxuegongweiminjiaoshouweibenwendegongtongtongxunzuozhe。boshishenghuangxueweibenwendediyizuozhe。gaiyanjiudedaokejibu、guojiazirankexuejijinyijiqifatiyudezizhu，shanghaitongbufusheguangyuan（ssrf）jiribentongbufusheguangyuan（spring-8）weigaiyanjiutigonglezhongyaodejishuzhichi。
cas12i2dejiegoushengwuxueyanjiu
a. cas12i2-crrna-dnasanyuanfuhewudezhengtijiegou
b. cas12i2deruvcjiegouyucuihuazhongxin
